Publication using two Agrisera antibodies (RbcL & ATPaseB) from Newmarket Scientific

Selected Paper:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4854705/

This publication used Anti-RbcL and Anti-ATPaseB antibodies from the Agrisera range. https://www.newmarketscientific.com/category/plant-research

Light Modulates the Biosynthesis and Organization of Cyanobacterial Carbon Fixation Machinery through Photosynthetic Electron Flow

Abstract

Cyanobacteria have evolved effective adaptive mechanisms to improve photosynthesis and CO₂ fixation. The central CO₂-fixing machinery is the carboxysome, which is composed of an icosahedral proteinaceous shell encapsulating the key carbon fixation enzyme, Rubisco, in the interior. Controlled biosynthesis and ordered organization of carboxysomes are vital to the CO₂-fixing activity of cyanobacterial cells. However, little is known about how carboxysome biosynthesis and spatial positioning are physiologically regulated to adjust to dynamic changes in the environment. Here, we used fluorescence tagging and live-cell confocal fluorescence imaging to explore the biosynthesis and subcellular localization of β-carboxysomes within a model cyanobacterium, Synechococcus elongatus PCC7942, in response to light variation. We demonstrated that β-carboxysome biosynthesis is accelerated in response to increasing light intensity, thereby enhancing the carbon fixation activity of the cell. Inhibition of photosynthetic electron flow impairs the accumulation of carboxysomes, indicating a close coordination between β-carboxysome biogenesis and photosynthetic electron transport. Likewise, the spatial organization of carboxysomes in the cell correlates with the redox state of photosynthetic electron transport chain. This study provides essential knowledge for us to modulate the β-carboxysome biosynthesis and function in cyanobacteria. In translational terms, the knowledge is instrumental for design and synthetic engineering of functional carboxysomes into higher plants to improve photosynthesis performance and CO₂ fixation.

This publication used Anti-RbcL and Anti-ATPaseB antibodies from the Agrisera range. https://www.newmarketscientific.com/category/plant-research