NextExpress Protein Expression Kit

The NextExpress Protein Expression Kit is a proprietary system for large-scale protein expression in eukaryotic cells. It provides superior expression of membrane proteins and high yields of cytoplasmic and secretory proteins.

The NextExpress Protein Expression Kit is also compatible with HEK293 and CHO cell expression systems, and is highly adaptable to commercial serum-free medium.



HEK 293F suspension cells were transfected with pDNA containing viral ORF (encoding a viral capsid protein p72 with transmembrane domains) using NextExpress™ Protein Expression Reagent. 72 hours post-transfection cells were lysed and membrane protein was detergent solubilised. Protein was purified using 2 step affinity and ion-exchange chromatography.

Key Features and Benefits:
• Unique system for robust expression and purification of membrane proteins in eukaryotic cells.
• 2 to 10-fold higher yield than other transfection reagents
• High cytoplasmic and secretory protein yields
• Compatible with HEK293 and CHO cell expression systems
• Simple, rapid protocol with fewer steps
• Easy to optimise due to excellent cell viability
• Superior adaptability in serum-free medium
• Significant cost saving compared to other reagents

Pricing is available on this link or for additional information or a copy of the datasheet please use our contact form or email us at tech@nktscientific.com.