20/03/2016

StressMarq - Antibodies

StressMarq primary antibodies cover many different research areas and are available conjugated to 17 different fluorescent dyes and enzymes.

At the moment conjugated primary antibodies are currently available at a 15% discount until the end of June 2016.

Some featured antibodies are listed below, however we have many more so please use the search box above to look for your specific antibody.

Stressmarq   antibodiesFeatured Antibodies:
Anti-HSP70
Anti-DNA/RNA Damage, 15A3
Anti-ENaC Beta
Anti-Aquaporin 4
Anti-Rhodopsin, 4D2
Anti-PSD95, 6G6
Anti-TLR4
Anti-Amyloid Oligomers (A11)
Anti-Nitrotyrosine, 39B6

Primary Antibody Conjugates Promotion
StressMarq conjugated primary antibodies are now available at a 15% discount until the end of June 2016. Quote SM616 on your order to get the discount. Questions? Contact us.

19/03/2016

Exosome Research

The HansaBioMed range provides essential tools for researchers in the field of exosome research, including the ExoTEST platform, a proprietary immune-based solution for capture, quantification and characterisation of exosomes from biological fluids.

Follow the links below to find further details of a specific HansaBioMed products. Or if you would like to request more information, please use our contact form.



Exosome Standards

Exosome capture and enrichment from biological fluids

EXO-Prep, Immunoplates and Immunobeads


Exosome Capture and Quantification from Biological fluids

All of the ExoTEST kits are also available as "Proteomic Test Kits" containing just 3 strips of wells instead of the 12 and a lower price for trial purposes or lower volume usage.

We can also provide custom-made ready-to-use kits for specific exosome immunocapture and quantification. Please use our contact form or email us with details of your requirements

FACS Analysis of Exosomes (Exo-FACS Kits)

Kits for DNA/RNA extraction from Exosomes

Exosome Related Antibodies

18/03/2016

NEXTflex Rapid DNA-Seq Library Prep Kits 2 hour protocol

The NEXTflex Rapid DNA-Seq Kit offers a streamlined 2 hour (or less) library prep protocol. Follow this link for more information on the NEXTflex Rapid DNA-Seq Kit or visit our NGS Page for details of the full range.

The NEXTflex™ Rapid DNA-Seq Kit (Part of the Bioo Scientific Next Generation Sequencing range) allows researchers to build sequencing-ready libraries for use on Illumina’s sequencing platforms starting with as little as 1 ng of DNA in two hours or less. This kit is ideal for use with genomic DNA, FFPE samples, ChIP DNA and low input clinical samples.

The NEXTflex Rapid DNA-Seq Kit is highly flexible in terms of sample requirements, accommodating a 3-log range of input DNA amounts from 1 ng to 1 µg. A bead-based, gel-free size selection protocol eliminates the need for agarose gels in size selection, and the availability of up to 96 unique adapter barcodes facilitates high-throughput applications. The NEXTflex Rapid DNA-Seq Kit incorporates “Enhanced Adapter Ligation Technology”, which facilitates ligation of long adapters, resulting in longer and more diverse sequencing reads. Bioo Scientific’s NEXTflex Ligation and Polymerase reaction mixes ensure the highest quality libraries for superior performance.

Flexible Multiplexing Options:
The NEXTflex™ Adapters are long, annealed adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes can be used with single, paired-end and multiplex reads. The NEXTflex Rapid DNA-Seq Kit is designed to be used with the NEXTflex™ and NEXTflex-96™ DNA Barcodes for 10 ng or more of starting material or the NEXTflex™ and NEXTflex-96™ ChIP-Seq Barcodes for less than 10 ng of starting material. These barcodes are available in sets of 6, 12, 24, 48 and 96 unique indexed adapters.

Key Points:
  • Flexible amounts of input DNA from 1 ng to 1 µg
  • Fast workflow requiring 2 hours or less, with minimal hands-on time
  • Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads
  • Creates libraries with less bias
  • Automation-friendly workflow is compatible with liquid handlers
  • Flexible barcode options - Up to 96 unique barcodes
  • Prepare single, paired-end and multiplexed genomic DNA libraries

Follow this link for more information on the NEXTflex™ Rapid DNA-Seq Kit or visit our NGS Page for details of the full Next Generation Sequencing range.

NGS Library prep kits and barcodes

Bioo Scientific NEXTflex Sequencing Kits & Barcodes provide enhanced adapter ligation giving the best ligation and amplification efficiency available in a library preparation kit. This ensures you maximise library diversity and see more of your sample in sequencing.

Nextflex Rapid Kits offer streamlined protocols with minimal hands on time, and have validated automation protocols for PerkinElmer's Sciclone NGS Workstation.
Nkt nextflex features apr14

15/03/2016

Validated automation protocols for PerkinElmer's Sciclone NGS Workstation for the NEXTflex DNA & RNA kits

We now have validated automation protocols for using our NEXTflex DNA-Seq and RNA-Seq library prep kits on PerkinElmer's Sciclone NGS Workstation. Follow the links below to view the our presentation or the protocols for each kit.

Presentation:
Protocols:
NEXTflex Rapid DNA-Seq Sciclone Automation Guide
NEXTflex Rapid Directional qRNA-Seq Sciclone Automation Guide
NEXTflex Rapid Directional RNA-Seq Sciclone Automation Guide
NEXTflex Rapid RNA-Seq Sciclone Automation Guide

If you have any questions or need further information or support please let us know by using our contact form or emailing us at tech@nktscientific.com.

For more information on the full range of Next Generation Sequencing library prep kits please visit our Next Generation Sequencing page.

HOT FIREPol EvaGreen qPCR Supermix

Solis BioDyne's new 5x HOT FIREPol EvaGreen qPCR Supermix includes a new, highly effi cient qPCR buff er which when combined with the EvaGreen dye, delivers a mastermix with truly impressive performance on any platform.

The Mix has eveything needed for your reaction. You just add the template and primers to provide you with highly sensitive qPCR.

Highly Sensitive and Effective qPCR:
• Superior sensitivity on low copy number targets
• UNG treatment capable due to dUTP addition
• Suitable for fragments up to 500 bp
• Suitable for GC-rich targets
• EvaGreen chemistry: Highly sensitive dye

PLUS:
No loss of activity should your polymerase be left on the bench over night!
Solis BioDyne polymerases are even shipped at room temperature so there are
NO
dry-ice shipping charges.

08/03/2016

NextExpress Protein Expression Kit

The NextExpress Protein Expression Kit is a proprietary system for large-scale protein expression in eukaryotic cells. It provides superior expression of membrane proteins and high yields of cytoplasmic and secretory proteins.

The NextExpress Protein Expression Kit is also compatible with HEK293 and CHO cell expression systems, and is highly adaptable to commercial serum-free medium.

NextExpress Protein Expression Kit

HEK 293F suspension cells were transfected with pDNA containing viral ORF (encoding a viral capsid protein p72 with transmembrane domains) using NextExpress™ Protein Expression Reagent. 72 hours post-transfection cells were lysed and membrane protein was detergent solubilised. Protein was purified using 2 step affinity and ion-exchange chromatography.

Key Features and Benefits:
Unique system for robust expression and purification of membrane proteins in eukaryotic cells.
2 to 10-fold higher yield than other transfection reagents
High cytoplasmic and secretory protein yields
Compatible with HEK293 and CHO cell expression systems
Simple, rapid protocol with fewer steps
Easy to optimise due to excellent cell viability
Superior adaptability in serum-free medium
Significant cost saving compared to other reagents

Pricing is available on this link or for additional information or a copy of the datasheet please use our contact form or email us at tech@nktscientific.com.