25/09/2017

EXO-DNAc-PS: Isolation of circulating and EV-associated DNA

Circulating DNA is emerging as a novel non-invasive tool for patient’s stratification and disease monitoring.

While most of the research has focused on circulating cell-free (cfDNA) or circulating-tumorcell-(CTC)-derived DNA, extracellular vesicle-(EVs)-associated DNA (EV-DNA) is emerging as a third valuable “liquid biopsy” platform.

Genomic single or double-stranded DNA and mitocondrial DNA have been detected in extracellular vesicles. In particular the majority of double-stranded DNA in extracellular vesicles seems to be associated with tumor derived exosomes (Thakur BK et al. 2014; Kalhert et al. 2014 ) where it represents the entire genome of the cancerous tumor from which exosomes were derived.

This discovery indicates the potential of exosomes as a possible diagnostic tool. Exosomes can easily be obtained from a simple blood sample, so combined with an equally simple method of isolation, they may provide an important diagnostic method in the future.


Get genomic DNA from exosomes with the EXO-DNAc Kit.

This kit combines the ability of our DNA-Prep reagent to co-isolate EVs and circulating DNA from biofluids or culture supernatants with a user friendly system for DNA purification. Isolated vesicles are simply lysed with the appropriate lysis buffer and DNA is purified by spin columns and optimised buffers with a fast turnaround time (approximatively 30 minutes).

Then finally, EXO-DNAc provides a DNA concentrator for concentrating the yield (4 fold concentration) and increasing the purity of the DNA to the levels required for digital PCR analysis.

Click here for further information on the Exo-DNAc Kits 




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