Publication using two Agrisera antibodies (RbcL & ATPaseB) from Newmarket Scientific

Selected Paper:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4854705/

This publication used Anti-RbcL and Anti-ATPaseB antibodies from the Agrisera range. https://www.newmarketscientific.com/category/plant-research

Light Modulates the Biosynthesis and Organization of Cyanobacterial Carbon Fixation Machinery through Photosynthetic Electron Flow

Abstract

Cyanobacteria have evolved effective adaptive mechanisms to improve photosynthesis and CO₂ fixation. The central CO₂-fixing machinery is the carboxysome, which is composed of an icosahedral proteinaceous shell encapsulating the key carbon fixation enzyme, Rubisco, in the interior. Controlled biosynthesis and ordered organization of carboxysomes are vital to the CO₂-fixing activity of cyanobacterial cells. However, little is known about how carboxysome biosynthesis and spatial positioning are physiologically regulated to adjust to dynamic changes in the environment. Here, we used fluorescence tagging and live-cell confocal fluorescence imaging to explore the biosynthesis and subcellular localization of β-carboxysomes within a model cyanobacterium, Synechococcus elongatus PCC7942, in response to light variation. We demonstrated that β-carboxysome biosynthesis is accelerated in response to increasing light intensity, thereby enhancing the carbon fixation activity of the cell. Inhibition of photosynthetic electron flow impairs the accumulation of carboxysomes, indicating a close coordination between β-carboxysome biogenesis and photosynthetic electron transport. Likewise, the spatial organization of carboxysomes in the cell correlates with the redox state of photosynthetic electron transport chain. This study provides essential knowledge for us to modulate the β-carboxysome biosynthesis and function in cyanobacteria. In translational terms, the knowledge is instrumental for design and synthetic engineering of functional carboxysomes into higher plants to improve photosynthesis performance and CO₂ fixation.

This publication used Anti-RbcL and Anti-ATPaseB antibodies from the Agrisera range. https://www.newmarketscientific.com/category/plant-research

NEXTflex™ Small RNA-Seq Kit v3

The NEXTflex™ Small RNA-Seq Kit v3 uses patented technology to provide a significantly reduced-bias small RNA library prep for Illumina sequencing platforms with both gel-free and low-input options.

Bioo Scientific'’s approach to reducing ligation-associated bias uses adapters with randomized bases at the ligation junctions, resulting in greatly decreased bias compared to standard protocols. This reduction in bias results in data that more accurately represents abundances of the small RNAs in the starting material.

In addition, this reduction of bias allows more miRNAs to be detected with fewer total reads, increasing efficiency and reducing cost for small RNA sequencing.

PAGE purification, required for traditional small RNA library prep, is tedious, time consuming, limits throughput, and prevents start-to-finish automation. The NEXTflex Small RNA-Seq Kit v3 allows for gel-free small RNA library prep.

This is possible thanks to the dual approach used for adapter-dimer reduction. Unprecedented reduction of adapter-dimer formation allows completely gel-free small RNA library prep when starting with ≥200 ng of total RNA. 

Libraries prepared with the NEXTflex Small RNA-Seq kit v3 have a higher proportion of reads mapping to miRNAs.

Watch our slideshare presentation below and download a copy of the manual here.

Reduced Bias Small RNA Library Prep with Gel-Free or Low-Input Options. 

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